1. Purification and Structure Solution of Heteromeric Acetyl-CoA Carboxylases from Gram (-) Bacteria Jeremy Lohman 2. Sample optimizations to enable the structure determination of biotin-dependent carboxylases Liang Tong 3. Measuring conformational dynamics in pyruvate carboxylase Martin St. Maurice 4. Characterization of guanidine carboxylases Jörg S. Hartig, Malte Sinn and Josefine Techel 5. Enzyme cascades for in vitro and in vivo FMN prenylation and UbiD activation Alexander Iakounine 6. Assessment of gamma-glutamyl carboxylase activity in its native milieu Jack Tie, Xuejie Chen and Darrel Stafford 7. Insights into the Methodology of Acetyl-CoA Carboxylase Inhibition Grover Waldrop 8. Cellular and Biochemical Approaches to Define GGCX Carboxylation of Vitamin K-Dependent Proteins Kathleen Berkner and Kurt William Runge 9. Mechanistic studies of the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase in the Wood-Ljungdahl pathway Stephen W. Ragsdale 10. Assessing the role of redox carriers in enzymatic CO2 reduction reactions Sean Elliott 11. UbiD-mediated enzymatic CO2 fixation David Leys 12. Computational methods for the study of enzyme-mediated carboxylation reactions Esteban Vöhringer-Martinez 13. In vitro Operation of Pyruvate:Ferredoxin Oxidoreductase for Biocatalytic Conversion of CO2 Hartmut Grammel 14. Evaluation of the Performance of Decarboxylases at Ambient and High CO2 Pressure Russ Hille 15. Measuring carbonic anhydrase activity in alpha-carboxysomes using stopped-flow Cecilia Blikstad 16. The activities of Rubisco and its regulatory proteins in leaf extracts Elizabete Carmo-Silva 17. Carboxylation in de novo Purine Biosynthesis Steven Firestine 18. Purification and characterization of biotin-dependent carboxylases from native tissues Martin St. Maurice

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