An Introduction to the Class Bunyaviricetes.- Use of Rift Valley Fever Virus Expressing NanoLuc Luciferase for the Assessment of Neutralizing Antibodies and Antivirals.- A Serological Assay for the Detection of Antibodies Generated Against California Serogroup Virus Infection.- Molecular Diagnosis of Phlebovirus riftense Infection Using an L-Segment Based Real-Time RT-PCR Method.- Ultracentrifugation Purification of Cache Valley Virus Using Iodixanol.- Cryogenic Electron Microscopy of Rift Valley Fever Virus.- Detection and Characterization of Antiviral Proteins against Bunyaviruses in Mosquito-Derived Cells.- Vector Competence Assays for RVFV in Mosquitoes.- Experimental Infection of Ticks with SFTSV.- Methods for Thrips Transmission and Maintenance of Tomato Spotted Wilt Virus (TSWV).- In Vitro Evaluation of Bunyavirus T-Cell Immunity.- Analysis of Leishbuviridae from Trypanosomatids.- Recombinant Protein Production and Purification of Rift Valley Fever Virus Nucleoprotein from Escherichia coli Expression Systems.- Bunyaviruses: Expression of Recombinant Gycoproteins from Insect Cells.- Murine Models to Study Crimean-Congo Hemorrhagic Fever Hepatic Injury.- A Review on Reliable and Standardized Animal Models to Study the Pathogenesis of Schmallenberg Virus in Ruminant Natural Host Species.- Schmallenberg Virus, From the Necropsy Room to the Laboratory.- Reverse Genetics System for Crimean-Congo Hemorrhagic Fever Virus.- Recombinant Vaccine Production: Production of a Recombinant CCHF MVA Vaccine.- Efficient Expression of Oropouche Virus Nonstructural Proteins NSs and Nsm.- Systemic Clodronate Liposome Treatment for Depletion of Blood-Contacting Phagocytes and Investigation of Their Role in the Control of Bunyavirus Viremia.

This volume explores the latest advancements in the study of bunyaviruses. The chapters in this book cover topics such as virus manipulation and purification; expression of recombinant proteins; the experimental study of bunyavirus infection in animals for virological and immunological analysis; and plant bunyaviruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Bunyaviruses: Methods and Protocols is a valuable resource for both expert and novice scientists who are interested in learning more about this important and developing field.