This book is based on two keywords: Bioradical and ESR. Research on the structure and function of bioradicals has been attrac­ ting growing attention in the field of biological science, and comprehensive investigations from many fields are helping to understand the real features of these species.

Preface.- Overview of bioradicals and ESR technology.- Chemistry of oxygen radicals.- Multiquantum ESR: Physics, technology and applications to bioradicals.- New ESR technologies.- Scanning tunneling microscopy (STM) methods for detecting ESR of a single spin center.- An open space ESR spectrometer and its application to high dielectric loss samples.- ESR imaging.- New experimental modalities of low frequency electron paramagnetic resonance imaging.- Whole body electron spin resonance imaging spectrometer.- Development of the rapid field scan L-band ESR-CT system.- Spin trapping.- Spin trapping.- History of spin trapping in Japan.- In vivo spin trapping.- In vivo ESR observation of bioradical metabolites in living animals.- Dithiocarbamate spin traps for in vivo detection of nitric oxide production in mice.- Spin labels.- Recent developments in spin labeling.- Electron spin resonance imaging of rat brain.- Metal complexes and metalloproteins.- ESEEM spectroscopy - probing active site structures of metalloproteins.- Nitric oxide, a versatile biological ligand for hemeproteins.- Antioxidants and foods.- Vitamin E and antioxidant interactions in biological systems.- Antioxidant activity of vitamin E and vitamin C derivatives in membrane mimetic systems.- Action of antioxidants as studied by electron spin resonance.- In vivo ESR measurements.- In vivo EPR spectroscopy.- Pharmaceutical aspects of ESR investigations on drug delivery systems, tissues and living systems.- In vivo ESR measurement of free radical reactions in living animals using nitroxyl probes.