Isolation of live immune cells from the tumour microenvironment by FACS.- In vitro evaluation of cancer cell immunogenicity and antigen-specific T-cell cytotoxicity by flow cytometry.- Retroviral transduction of human primary T cells followed by real-time T-cell mediated cancer cell cytolysis analysis.- Expansion and retroviral transduction of primary murine T cells for CAR T cell therapy.- In Situ Decellularisation of Tissues applied to the topographical analysis of tumour associated extracellular matrix.- Monitoring cell cytoskeleton variations upon piezoelectric stimulation: Implications for the immune system.- Preparation method and in vitro characterization of nanoparticles sensitive to tumor microenvironment.- A new microfluidic device to facilitate functional precision medicine assays.- Kinetic detection of apoptosis events via caspase 3/7 activation in a tumor-immune microenvironment on a chip.- Hypoxic 3D Tumor Model for Evaluating of CAR-T Cell Therapy In Vitro.- Rapid screening of CAR T cell functional improvement strategies by highly-multiplexed single-cell secretomics.- Genome editing in CAR-T cells using CRISPR/Cas9 technology.- Genetic modification of tumor-infiltrating lymphocytes, peripheral T cells, and T cell model cell lines.- Transposon-based manufacturing of human CAR-T cells.- Redirecting human conventional and regulatory T cells using chimeric antigen receptors.- How to Test Human CAR T cells in Solid Tumors, The Next Frontier of CAR T Cell Therapy.- Nano-Optogenetic CAR-T Cell Immunotherapy.- A non-viral piggyBac transposon-mediated method to generate large-scale CAR-NK cells from human peripheral blood primary NK cells.- Engineering probiotic E.coli Nissle 1917 for release of therapeutic nanobodies.

This volume details multiple areas of new and emerging methods to develop the next generation of immunotherapy treatments. Chapters guide readers through analysis and characterisation of the interactions between tumour and immune cells, and cell engineering tools for cancer treatment, to provide a unique and compelling set of techniques instrumental to work with, and engineer, immune cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Cancer Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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