Autoantibodies as Diagnostic Tools.- Non-specific Binding in Immunoassays for Autoantibodies.- Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on HEp-2 Cells.- Detection of Anti-Neutrophil Cytoplasmic Antibodies by Indirect Immunofluorescence.- Determination of Subset-Restricted Anti-Neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry.- Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry.- Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry.- Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay.- Determination of Autoantibodies to Salivary Gland Antigens.- Measurement of autoantibodies to the gastric H+,K+-ATPase (ATP4A/B) using a Luciferase Immuno Precipitation System (LIPS).- Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay.- Determination of Autoantibodies to Neutrophil Granule Constituents by Solid Phase Immunoassay.- Determination of Autoantibodies to C3 Nephritic Factors by ELISA.- Determination of Anti-C1q Autoantibodies by Western Blot.- Anti-Factor H Autoantibodies Assay by ELISA.- Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay.- Determination of CRP Autoantibodies by SPR Immunoassay.- Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis.- Detection of SSA and SSB Autoantibodies by Enzyme-Linked Immunosorbent Assay.- Assessment of Peptidylarginine Deiminase Activity by ELISA using Human Fibrinogen as Substrate.- The Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay.- Determination of Autoantibodies by Line Blotting.- Determination of Rheumatoid Factors by ELISA.- Detection of autoantibodies to complement components by surface plasmon resonance-based technology. 

This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients.