Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production.

Basic Techniques for Establishing Production Cell Lines.- Transduction Technologies.- Cells for Gene Therapy and Vector Production.- Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments.- DNA Fingerprinting and Characterization of Animal Cell Lines.- Basic Cultivation Techniques.- Microcarrier Cell Culture Technology.- Cell Encapsulation.- Tools for High-Throughput Medium and Process Optimization.- Cell Characterization and Analysis.- Cell Counting and Viability Measurements.- Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry.- Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors.- Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture.- Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation.- Measurement of Apoptosis in Cell Culture.- Metabolic Flux Estimation in Mammalian Cell Cultures.- Special Cultivation Techniques.- Disposable Bioreactors for Inoculum Production and Protein Expression.- Hollow Fiber Cell Culture.- Cultivation of Mammalian Cells in Fixed-Bed Reactors.- Configuration of Bioreactors.- Downstream Techniques.- Membrane Filtration in Animal Cell Culture.- Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins.- Special Applications.- Vaccine Production.- Retrovirus Production and Characterization.- Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production.

Chapters written by world-renowned experts Details novel techniques for basic cultivation and complex techniques such as disposable bioreactors, hollow fiber cell culture and fixed bed reactors Provides a state-of-the-art manual for setting up mammalian cell lines for production of biopharmaceuticals Divided into sections to mimic the processes required for different stages of production

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