The present book delves into the intricacies of biofilm formation across diverse industries, including food, agriculture, soil and plant health, fruits and vegetables, seafood, dairy and milk products, meat and meat products, and beverages. It offers an in-depth explanation of biofilm formation mechanism and assaying. Moreover, the book explores various techniques and approaches that interfere with bacterial attachment, bacterial communication systems, and biofilm matrices. It also discusses the role of biofilm communication and quorum sensing, the impact of antibiotics on biofilm formation, biofilm inhibitory peptides, and novel methods of biofilm inhibition. The book examines both the positive and negative aspects of biofilms, including strategies for regulating harmful bacterial biofilms and mechanisms of formation. Additionally, it highlights future perspectives on bacterial biofilms and the use of dietary phytochemicals and removal of bacterial adhesion to inhibit biofilms. Another significant aspect of the book is the release of antimicrobial compounds, including bacteriocins, bacteriophages, enzymes, essential oils, nano emulsions, and nano particles, to prevent biofilm formation. The book also explores newer techniques for removing biofilms, such as Cold Plasma Technology, Ultra Sound, Light-assisted Technologies, Pulse Electric Field, and High-Pressure Processing. Furthermore, the book discusses the impact of genetic diversity on biofilm formation and its implications for human health and the environment. It is designed for readers, industrialists, students, and society activists who are interested in maintaining a green and clean environment, safe food, and good health. The book offers a comprehensive review of the subject, ranging from the introduction to future implications on biofilms. It provides the most recent updates in the field with a holistic approach, aiming to equip readers with the necessary information to manage biofilm and ensure hygienic practices and food safety.